Cell tracking

The very high cell compatibility of CelLuminate™ Red allows the delivery of large amounts of fluorophores. Provided the delivered fluorophore is not cytotoxic, CelLuminate Red can be employed to fluorescently track cells in 2D and 3D environments for extended periods

1 Day

7 Days

14 days

Human dermal fibroblasts (HDFs) were incubated with Rhodamine-loaded CelLuminate Red for 24 hours. The cells were subsequently washed with PBS several times and placed in fresh media. The fluorescence micrographs and the fluorescence intensity decay readings show that cells incubated with CelLuminate Red can retain the fluorophore for extended periods

Extended fluorescence intensity with CelLuminate

Non-cytotoxic

% HDF cell viability over time

Real-time monitoring

CelLuminate enables real-time monitoring of cell migration and organisation within a matrix.

Human dermal fibroblasts (HDFs) seeded at one end of the pre-cast fibrin gel using a fibrin clot cell seeding method in 6-well tissue culture plate were stained with CelLuminate Red. They were monitored using fluorescence microscopy after static culture in DMEM with 10% FCS for 7 days (A, B) and 14 days (C, D) respectively. Fluorescent (red) micrographs show HDFs in the initial cell-seeding fibrin clot (A, C) and after migration into the border area between the initial cell-seeding area and the pre-cast gel (B, D).

Tissue-engineered human oral epithelium imaged by confocal laser-scanning microscopy. Primary human oral keratinocytes were previously stained with rhodamine-loaded CelLuminate (white) placed on top of a collagen gel (autofluorescence-blue) at the air-liquid interface, and imaged after 10 days to allow differentiation and epithelium formation. X-Y top view (A), Z-X section (B), computer- reconstructed 3D bottom view ©, and 3D top view (D) of the epithelium formed by the keratinocytes.