Sub cellular targeting

Due to its polymer technology, CelLuminate™ Red nanoparticles can be loaded with water-soluble, water-insoluble, and amphiphilic molecules. This allows the delivery of a large variety of fluorophores within cells and consequently the selective staining of specific sub-cellular compartments and components or biological molecules.

Sphyngolipids

Live MLO-A5 cells stained with CelLuminate delivering BODIPY® FL C5-ceramide

Live MLO-A5 cell stained with CelLuminate™ delivering BODIPY® FL C5-ceramide

Cholesterol

Live primary human dermal fibroblast (HDF) cells stained with CelLuminate delivering NBD cholesterol

Live primary HDF cells stained with CelLuminate™ delivering NBD cholesterol

DNA

Live primary HDF cells stained with CelLuminate delivering Propidium Iodide

Live Primary HDF cells stained with CelLuminate™ delivering Propidium Iodide

Antibodies

Live primary HDF cell stained with CelLuminate delivering Alexa546-IgG

Live primary HDF cell stained with CelLuminate™ delivering Alexa546-IgG

Phospholipids

Live primary HDF cell membranes stained with CelLuminate delivering Fluorescein DHPE

Live primary HDF cell membranes stained with CelLuminate™ delivering Fluorescein DHPE

Confocal laser scanning microscopy 3D reconstruction of live HDF filopodias stained with CelLuminate delivering Rhodamine B octadecyl ester

Confocal laser scanning microscopy 3D reconstruction of live HDF filopodias stained with CelLuminate™ delivering Rhodamine B octadecyl ester

3-D confocal microscope reconstruction of a single primary HDF


Details of a live HDF cell perinuclear and nuclear areas

Details of a live HDF cell perinuclear and nuclear areas

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Pictures and data courtesy of Dr G Battaglia and Ms M Massignani, University of Sheffield, UK