http://celluminate.dmlogic.net/news/ en info@celluminate.com Copyright 2010 2010-05-24T07:47:11+00:00 http://www.celluminate.com/blog/article/fluorescence_imaging_publication/ http://www.celluminate.com/blog/article/fluorescence_imaging_publication/#When:06:47:11Z A paper describing the use of CelLuminate is now available on line in the Public Library of Science at the following link: http://dx.plos.org/10.1371/journal.pone.0010459 2010-05-24T06:47:11+00:00 http://www.celluminate.com/blog/article/celluminate_vps/ http://www.celluminate.com/blog/article/celluminate_vps/#When:10:20:15Z As we’ve rolled out CelLuminate Red across the world, we’ve had numerous requests for empty nanoparticles so our customers can load whatever they want. Now we've decided to make that product available and we've called it CelLuminate VPS for Vesicle-forming Polymer System. The product comes as a powder for our customers to make their own CelLuminate vesicles encapsulating whatever they want for delivery into live cells. 2009-07-24T10:20:15+00:00 http://www.celluminate.com/blog/article/dr_paul_townsend_discusses_celluminate/ http://www.celluminate.com/blog/article/dr_paul_townsend_discusses_celluminate/#When:11:16:27Z CelLuminate is a very user-friendly cell stain which has been used to stain a range of mammalian cells from human, rat and mouse origin without need for lots of optimisation. With variation of the staining time we can use CelLuminate to stain cells showing their cytoplasm clearly and no nuclear staining (after approximately 3 hours) or we do see discrete pockets of staining when the dye is administered in the media for periods of overnight or longer. The aspect we like most like about CelLuminate is that in human cells the stain can be applied for long periods without effects on cell viability. Additionally, with removal of the stain from the media cells remain stained at high intensity for up to 5 days. This, we predict, would be very useful for in vivo staining. Overall, the variety of cells we have tested (human leukaemias, human, mouse and rodent primary cells) all stain well with CelLuminate - that's a definite positive versus our past experience with other cell stains! 2009-05-22T11:16:27+00:00 http://www.celluminate.com/blog/article/how_i_use_celluminate/ http://www.celluminate.com/blog/article/how_i_use_celluminate/#When:05:25:09Z Celluminate is extremely versatile. I use it routinely to label any type of cells without loss of viability or activation of cellular stress/differentiation pathways. I have successfully labelled many different (and challenging) cell types such as, neutrophiles, Stem cells, neurons, tumoral cells, primary dermal/epidermal cells. It is a perfect tool for bioimaging. Because it takes advantage of the cellular endocytic pathway and it escapes the endosomal route, it diffuses very nicely inside the cell and somehow, this results in a fantastic resolution for imaging cellular processes and filopodia. I have always had problems with other dyes for live cell imaging, they tend to accumulate in granular/patchy areas which makes complicated to visualise the cellular borders (especially at high magnification in confocal microscopy). This is not the case with Celluminate. Also, the levels of uptake are so high that I never get the background noise. This is especially important for my 3D tissue engineered construct bioimaging or solid tumour mass imaging. The diffusion through is fantastic and you do not get the typical background noise of the cfse dyes. You can track the cells all the way through different tissue layers. It is great for cancer invasion analysis. The cells keep the same signal for a long period of time in culture so it is ideal for time lapsbioe microscopy. The fact that Celluminate™ does not depend on intracellular enzymatic reactions to be fluorescent is very important, this gives a uniform labelling of the cellular population (no matter how long the cells have been stained for) which is great when you are trying to do FACS analysis. Last but not least, it is so easy to use that you can combine it with any experimental methodology: you can use it in cell suspension or in adherent monolayers, at any type of cell density, in medium or PBS, with or without serum, in 2D or 3D, long or short period of incubation, multiple additions.. no titrations, no previous dilution steps, no cell harming/permeating substances; just direct from the package, into the cells and ready to image. 2009-05-07T05:25:09+00:00