CelLuminate is a very user-friendly cell stain which has been used to stain a range of mammalian cells from human, rat and mouse origin without need for lots of optimisation. With variation of the staining time we can use CelLuminate to stain cells showing their cytoplasm clearly and no nuclear staining (after approximately 3 hours) or we do see discrete pockets of staining when the dye is administered in the media for periods of overnight or longer.
The aspect we like most like about CelLuminate is that in human cells the stain can be applied for long periods without effects on cell viability. Additionally, with removal of the stain from the media cells remain stained at high intensity for up to 5 days. This, we predict, would be very useful for in vivo staining.
Overall, the variety of cells we have tested (human leukaemias, human, mouse and rodent primary cells) all stain well with CelLuminate - that's a definite positive versus our past experience with other cell stains!
